Campylobacter jejuni heme oxygenase ChuZ promotes inflammasome activation
ZHANG Rui
XIONG Yisong
LIU Yuan
JIANG Zhongyong
CHANG Kai
HU Zonghai
XIONG Jie
Abstract：This study aimed to explore the mechanism that Campylobacter jejuni heme oxygenase ChuZ promotes NLRP3 inflammasome activation.Mouse bone marrow was isolated and differentiated to bone marrow-derived macrophage (BMM),and then stimulated with WT C.jejuni,△ ChuZ C.jejuni or media.The expression of IL-1β,NLRP3,ASC,procaspase-1 and caspase-1 were detected by qRT-PCR,ELISA and Western blotting,respectively.Compared with control group,WT C.jejuni,△ ChuZ C.jejuni significantly enhenced the expression levels of IL-1β,NLRP3,procaspase-1 and caspase-1 in BMM cells;compared with WT BMM group,the protein level of IL-1β significantly decreased in NLRP3-/-BMM cells stimulated with WT C.jejuni or △ ChuZ C.jejuni.Meanwhile,MCC950 (NLRP3 inhibitor) inhibited IL-1β secretion level in BMM cells stimulated with WT C.jejuni,or △ ChuZ C.jejuni.Taken together,Campylobacter jejuni heme oxygenase ChuZ can promote IL-1β expression,and is related to NLRP3/ASC/caspase-1 inflammasome activation levels,thus may take part in the development and progression of Campylobacterjejuni-induced inflammation.
Keywords：Campylobacter jejuniChuZInflammasomeIL-1βNLRP3
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 955-959 )