Anti-tumor effects of CIK on SGC-7901 gastric xenograft in mouse model

HUANG Wei; XIANG Zheng; ZHANG Caiquan

doi:10.13431/j.cnki.immunol.j.20170166

Abstract：To investigate the anti-tumor effect of cytokine-induced killer cells (CIK) on gastric xenograft in mice and its mechanism,60 C57BL/6 mice were selected and divided randomly into model control group,5-Fu group,CIK high dose group,CIK middle dose,CIK low dose group and normal control group (n=10).After treatnent of 4 weeks,the levels of MMP-9,VEGF and bFGF in serum and gastric xenograft of mice were measured.Data showed that the tumor volume in CIK middle dose group was significantly decreased compared with that in CIK low dose group at 1 week,2 weeks,3 weeks and 4 weeks after treatment (P＜0.05);the tumor volume in CIK high dose group was significantly decreased compared with that in CIK middle dose group at 1 week,2 weeks,3 weeks and 4 weeks after treatment (P＜0.05).The levels of MMP-9,VEGF and bFGF in serum of CIK middle dose group were significantly decreased compared with those of CIK low dose group (P＞0.05) 4 weeks after treatment (P＜0.05);the levels of MMP-9,VEGF and bFGF in serum of CIK high dose group were significantly decreased compared with those of CIK middle dose group (P＞0.05) 4 weeks after treatment (P＜0.05).The mRNA and protein levels of MMP-9,VEGF and bFGF in the gastric xenograft of mice in CIK middle dose group were significantly decreased compared with those in CIK low dose group (P＞0.05) 4 weeks after treatment (P＜0.05);the mRNA and protein levels of MMP-9,VEGF and bFGF in the gastric xenograft of mice in CIK high dose group were significantly decreased compared with those in CIK middle dose group (P＞0.05) 4 weeks after treatment (P＜0.05).Taken together,cytokine-induced killer cells can significantly inhibit the growth of SGC-7901 gastric xenograft in mouse model and can decrease the expression of MMP-9,VEGF and bFGF in blood and cancer tissue.