The effect of NF-κB/p65 silencing on the expression of NOX1 gene in TNF-α-induced alveolar epithelial cells
WU Weijing
ZHANG Jiamin
ZENG Yiming
LI Li
HUANG Wenjie
Abstract：This study was performed to establish an in vitro inflammatory cell culture model of acute lung injury,and observe the expression of NOX1 gene and oxidative stress induced by TNF-α,and to investigate the effects of NF-κB/p65 on NOX1 by silencing NF-κB/p65 gene.A549 cells were treated with RNAi to down-regulate the expression of NF-κB,then treated with TNF-oα (10 ng/ml).RT-PCR and Western blotting were performed to analyze the expression of NOX1 gene.The concentration of ROS was detected by DCFH-DA probe assay,and the contents of MDA,total antioxidant capacity,SOD and GSH were measured by colorimetric method.Data showed that the transcription and translation of NF-κB/p65 were significantly up-regulated in A549 cells stimulated by TNF-α (P＜0.05).The levels of ROS and MDA were improved,total antioxidant capacity,SOD and GSH were decreased markedly (P＜0.05).NF-κB/p65 RNAi could down-regulate the expression of NOX1 gene and the oxidative stress injury,reduce the levels of ROS and MDA,meanwhile,total antioxidant capacity,SOD and GSH were increased dramatically (P＜0.05).In conclusions,NF-κB/p65 silencing could decrease the expression of NOX1 gene and reduce oxidative-stress stimulated by TNF-α,suggesting that NF-κB/p65 may play an essential role in the expression of NOX1 gene.
Keywords：NF-κB/p65NOX1 geneOxidative stressAcute lung injury
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 943-948 )