The expression pattern of cytokines in mesenchymal stem cells and macrophages co-cultured system
GAO Hongfei
ZHANG Qian
CHEN Long
SONG Gongyu
ZHAO Yujie
WAN Weihong
LIU Zulin
YU Limei
ZHANG Tao
Abstract：To investigate the changes of cytokines expression in macrophages (M0) cocultured with blood-derived mesenchymal stem cells (PBMSCs) or bone marrow-derived mesenchymal stem cells (BMMSCs),rat PBMSCs,BMMSCs and macrophages were isolated,cultured,and identified,and 5 groups were set as PBMSCs,BMMSCs,macrophages,PBMSCs co-cultured with macrophages (PBMSCs+M0),BMMSCs co-cultured with macrophages (BMMSCs+M0).All cells were cultured for 3 days and then the supernatant was collected to detect the concentration of cytokines IL-10,IFN-γ,IL-1β and TNF-α by Bio-Plex immune-microsphere assay.Macrophages co-cultured with PBMSCs/BMMSCs showed significantly higher levels of IL-10 and IL-1 β (P＜0.05),lower level of TNF-α in the supernatant (P＜0.05),and unchanged level of IFN-γ(P＞0.05),as compared with PBMSCs/BMMSCs.The changes of all cytokines in BMMSCs+M0 group were more significant,as compared with PBMSCs+M0 group (P＜ 0.05).In conclusion,co-culture of PBMSCs/BMMSCs with macrophage can stimulate the secretion of IL-10 and IL-1β,reduce the secretion of TNF-α,and the inflammatory modifying ability of BMMSCs is stronger that that of PBMSCs,which provides a new strategy for the application of mesenchymal stem cells in different disease states.
Keywords：Peripheral bloodMesenchymal stem cellsMacrophagesTranswell co-culture systemCytokines
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 930-936 )