Allicin inhibits microglia activation and exerts anti-inflammatory effects in cerebral ischemia reperfusion model

JIANG Yunchuan

doi:10.13431/j.cnki.immunol.j.20170149

Abstract：To investigate the effects of allicin on microglia activation and the release of inflammatory cytokines by microglia in the model of cerebral ischemia reperfusion of rats,middle cerebral artery occlusion (MCAO) model was used with reperfusion after 30 minutes ischemia.The activation of microglia (with the marker IBA-1) and the expression of interleukin 1-β(IL-1β) in ischemic cortex at first and third day were detected by Western blotting.The protein and mRNA levels of IBA-1 and IL-1β were detected by Western blotting and qPCR in ischemia and reperfusion model after caudal vein injection of garlicin.In vitro,BV2 cells were pretreated with allicin and then subjected to oxygen glucose deprivation (OGD),and then the protein and mRNA levels of IL-1β were detected by Westem blotting and qPCR.Data showed that the level of activation microglia and IL-1β expression in ischemic cortex increased significantly at first and third day (P＜0.05) in the rat model of ischemia-reperfusion.After the treatment of allicin in the ischemic reperfusion model,the protein and mRNA levels of IBA-1 and IL-1β in ischemic cortex decreased significantly (P＜0.05).In vitro,the protein and mRNA levels of IL-1β decreased significantly (P＜0.05) in BV2 cells pretreated with allicin and treated with OGD.In the model of cerebral ischemia and reperfusion,allicin may exert an anti-inflammatory effect by inhibiting the activation of microglial cells and suppressing the release of IL-1 β by microglial.