Effects of compound Cordyceps militaris on small intestine inflammation and the survival rate of IEC-6 cells
XU Huilin
CHEN Mengqiu
PAN Suhua
LI Hao
Abstract：This study was performed to explore the intervention effect of compound Cordyceps militaris (CCM) on small intestinal inflammation induced by high-fat and on IEC-6 cell apoptosis induced by LPS and H2O2.The C57BL/6J mouse model of small intestinal inflammation was induced with high-fat diet,followed by oral administration of 90,180 or 540 mg· kg-1· d-1 CCM,once a day for 8 weeks.Then we observed the levels of total cholesterol (TG),triacylglyceride (T-CHO),low density lipoprotein cholesterol (LDL-C),high density lipoprotein cholesterol (HDL-C),tumor necrosis factor-α (TNF-α),interleukin-1β (IL-1β),interleukin 6 (IL-6),lipopolysaccharide (LPS) in serum,as well as pathological changes in jejunum and ileum tissue.The survival rate of IEC-6 cells induced by H2O2 and LPS was determined by MTT method.Compared with the model group,CCM groups showed obvious relieves in inflammatory infiltration in mouse jejunum and ileum,significant reduction in T-CHO,LDL-C,TNF-α,IL-1β,IL-6 and LPS in serum,and CCM can increased cell survival rate significantly.In conclusion,CCM can improve mouse small intestine inflammation induced by high fat,and the mechanism may associate with the inhibition of small intestine epithelial cells apoptosis.
Keywords：Compound Cordyceps militarisSmall intestine inflammationIEC-6 cellsCytokines
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 783-788,794 )