Chlamydia trachomatis plasmid-encoded Pgp3 induces IL-8 production in HeLa cells through NOD1/RIP2 signaling pathway
ZHANG Li
QIAN Hou
XU Yingjie
ZOU Yan
LI Zhongyu
CHEN Chaoqun
Abstract：To investigate the mechanism of Chlamydia trachomatis plasmid-encoded Pgp3 inducing the production of IL-8 in HeLa cells,several concentrations of Pgp3 were selected to stimulate HeLa cells in different time course,and then the IL-8 expression level,NOD 1 and RIP2 transcriptional levels were detected via real-time PCR and Western blotting.Besides,Western blot was used to detect ERK1/2 and p38 phosphorylating on various time course.Data showed that Pgp3 elicited IL-8 production in HeLa cells in a way of time and concentration dependence,and the optimal concentration and time was 24 μg/ml and 24 h,respectively;Pgp3 could modulate the expression of NOD1/RIP2 and phosphorylate ERK1/2 and p38,which would not affect by pretreatment of NOD1 inhibitor ML130,RIP2 inhibitor GSK583.And NOD1 inhibitor ML130,RIP2 inhibitor GSK583,ERK inhibitor PD98059 or p38 inhibitor LY2228820 could reverse the up-regulation of IL-8 induced by Pgp3 stimulation.Taken together,Pgp3 protein induces IL-8 production in HeLa cells through NOD1/RIP2 in ERK or p38-independent pathway.
Keywords：Chlamydia trachomatisPlasmid glycoprotein 3 (Pgp3)IL-8NOD1/RIP2ERK
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 760-764 )