Generation of transgenic mice expressing neo-autoantigen OVA exclusively in skeletal muscle
HUANG Tao
DING Maochao
SHI Dandan
GU Ruicai
XIAO Jiangwei
GUO Mengxia
LI Junhua
LIAO Hua
Abstract：To construct a transgenic mouse model expressing neo-autoantigen OVA exclusively in skeletal muscle,we inserted MCK3E-OVA-pA into mouse genome randomly by PiggyBAC translocation enzyme system and fertilized egg injections.PiggyBAC mRNA was obtained by transcription in vitro,then the PiggyBAC mRNA and a plasmid containing chicken OVA fragments,skeletal muscle creatine kinase promoter (MCK),boot sequence H-2Kb and transmembrane sequence H-2Db were microinjected into the fertilized eggs of C57BL/6 J mice to get founder mice.Tg mice was crossed with wild B6 mice.PCR and Western blot detections confirmed the expression of OVA in skeletal muscle is exclusive.PCR analysis demonstrated that the OVA mRNA was expressed in muscle tissue of Muscle-OVA Tg mice,while Western blot confirmed that OVA protein was expressed exclusively in skeletal muscle,other tissue/organ,such as the heart,spleen,lung and kidney tissues were negative.Taken together,a muscle-OVA Tg mouse model has been generated by using PiggyBAC translocation enzyme system,which could be used for study of immune response of skeletal muscle.
Keywords：OvalbuminPiggyBACTransgenosisSkeletal muscle
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 733-736 )