The influence of leptin on regulatory T cells in murine mammary carcinoma model
LIU Wenhui
ZHOU Xinyi
ZHANG Jie
LIU Jing
LUO Naixiang
Abstract：To investigate the effect of leptin on regulatory T cells in murine mammary carcinoma model,we cultured 4T1 breast cancer cells and established murine mammary carcinoma models bearing these cells,and randomly divided them into control and experimental groups.The experimental group was treated with leptin,while the control group was treated with the same volume of normal saline.Then the change of tumor size was monitored;the percentage of regulatory T cells in peripheral blood,tumor draining lymph node and spleen were measured by FACS;the content of TGF-β1/IL-10 was detected by ELISA.Compared with control group,leptin promoted the tumor growth,up-regulated the percentage of T regulatory cells in peripheral blood and spleen,and raised the content of TGF-β1/IL-10 in serum in a dose-dependent manner (P＜0.05),while did not effects the level of regulatory T cells in tumor draining lymph node.Taken together,leptin can up-regulate the percentage of T regulatory cells,suppress the anti-tumor immunity of organism and promote the tumor growth.
Keywords：Breast cancerLeptinT regulatory cells
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 678-682 )