Effects of bone marrow mesenchymal stem cells therapy on immunological function in liver cirrhosis rats

XIA Jinhua; XIA Jianchuan; LIN Wanfei; XIE Liyan; LIAO Chuanying

doi:10.13431/j.cnki.immunol.j.20170111

Abstract：This study performed to investigate the effect of bone marrow mesenchymal stem cells on immunocyte in immune organ of liver cirrhosis rat,by detecting the proliferation of lymphocyte,expression of Foxp3 mRNA and the level of CD4+CD25+ Foxp3+T cell,thereby explaining the mechanism of immunoloregulation of bone marrow mesenchymal stem cells in vivo.Total of 150 male Wistar rats were randomly divided into 3 groups:the control group,the liver cirrhosis group,and the treatment group.And then the proliferation of lymphocyte,the expression of Foxp3 mRNA and the level of CD4+CD25+Foxp3+T cell were detect in all groups.Microscopy showed that the liver cells were arranging tidy,and there were no fiber hyperplasia and no false lobulation in normal group;while in the model group,the construction of the normal hepatic lobufe was broken,and the liver was separated by the fibrous bands into false lobulation,and inflammatory cell infiltration could be seen around the area of converging pipes the liver antrum and central vein;as to the treatment group,the hydropic degeneration of liver cell was obviously alleviated,and there was a little amount of hyperplastic fiber.Data also showed that the proliferation of spleen T and B lymphocyte was higher in the liver cirrhosis group than that in the control group (P＜0.05),and down-regulated after treatment (P＜0.05);the expression of Foxp3 mRNA was lower in the liver cirrhosis group than that in the normal control (P＜0.05),and up-regulated in treatment group (P＜0.05);the level of CD4+CD25+ Foxp3+Treg cell was lower in the liver cirrhosis group than that in the normal control (P＜0.05),and up-regulated in treatment group (P＜0.05).In conclusion,the mechanism of bone marrow mesenchymal stem cell therapy to liver cirrhosis rats maybe relate to inhibiting the abnormal proliferation of lymphocyte and promoting the level of Foxp3 mRNA and CD4+CD25+Foxp3+Treg cell.And the early treatment is more effectively.