The expression of LAIR-1 receptor in LPS-stimulated RAW264.7 cells
XIN Wenyu
ZHANG Dan
WANG Qiaoyun
Abstract：The purpose of the present study is to investigate the expression changes of LAIR-1 receptor in RAW264.7 cells induced by LPS.The cells were stimulated with LPS for 0,6,12,24 or 48 h.Then the content of NO was assayed by Greiss method,the concentrations of TNF-α and IL-6 in the culture supernatant were analyzed by ELISA,and the expression of LAIR-1 receptor was determined with Western blot.Also,the cells were pre-treated with NF-κB inhibitor PDTC (50 μmol/L) for 1 h and were then cultured with LPS for 24 h followed by detection of LAIR-1 receptor.Data showed that expression of LAIR-1 decreased gradually when the production of NO,TNF-α and IL-6 increased in RAW264.7 cells stimulated by LPS for different time.When the cells pre-treated with PDTC,the expression of LAIR-1 was higher than that of LPS alone group.Taken together,LAIR-1 would declined rapidly in LPS-activated macrophages,and up-regulated when NF-κB pathway inhibited,suggesting LAIR-1 is closely related with the development of inflammation.
Keywords：InflammationRAW264.7 cellLAIR-1 receptor
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 575-579 )