Preliminary study on the effect of Periplaneta americana peptide on immune of MFC tumor bearing mice

CHANG Xu; WANG Cong; OU Hongli; ZHAO Wei; WU Xiumei; ZHANG Chenggui; LIU Guangming; BAI Li

doi:10.13431/j.cnki.immunol.j.20170100

Abstract：This article makes a preliminary exploration in the effect of Periplaneta american peptides on immunity in MFC bearing mice to investigate the anti-tumor mechanism of the peptides.We established a subcutaneous tumor model in Balb/c mice,and divided them into 5 groups:model group,CTX group,the Periplaneta americana peptide complex C Ⅱ-3 high and low dose groups,synthetic peptide HFDT1 group,and normal Balb/c mice were used as normal control group.The model group and the normal group were given normal saline by gavage,while other groups were administrated with corresponding drugs.Ten days after treatment,the data in the mice were analyzed.The result showed that the high and low dose C l-3,synthetic peptide HFDT1 and CTX inhibited the MFC tumor growth in Balb/c mice.Compared with CTX,the high and low dose C Ⅱ-3,and synthetic peptide HFDT1 increased the mouse weight,and the numbers of leukocytes,lymphocyte,monocyte and granulocyte of peripheral blood (P＜0.05).C Ⅱ-3 and HFDT1 also increased the spleen index and thymus index,the number of splenic lymphocyte,and the ratio of NK cells.Compared with model group,the ratio of T cells in the spleen was increased in C Ⅱ-3 and HFDT1 groups.The above results indicated that the Periplaneta americana peptide complex C Ⅱ-3 and peptide HFDT1 have tumor-suppressing capabilities.The anti-tumor effect of the peptides maybe achieved through promoting the immunity with non-side effect.Further research is needed to study the anti-tumor mechanism of Periplaneta americana peptides.The peptides may become a strong anti-tumor candidate with low side effect.