The significance of circulating microRNAs detection in diagnose and therapeutic surveillance of rat ulcerative colitis

doi:10.13431/j.cnki.immunol.j.20170094

Abstract：This study aimed to explore and establish a preclinical strategy for diagnosis and therapeutic following of rat ulcerative colitis based on circulating microRNA.First stage:rat ulcerative colitis was induced by dextran sulfate sodium (DSS).Differentially expressed serum microRNAs were screened and validated by review of literature and real-time RT-PCR,while serum pANCA was tested by ELISA.Then combinative diagnostic Indice was established using logistic regression model based on the data from first stage.The diagnostic performance,including AUC,specificity,sensitivity and Youden index,were evaluated by receiver operating characteristic (ROC) analysis.Second stage:the diagnostic performance of combinative indice were validated in this stage,and the indice was further used for evaluation of therapeutic effect on ulcerative colitis.Data showed that rat ulcerative colitis model was successfully established.MiR-20b,miR-21 and let-7e* were up-regulated in the serum of ulcerative colitis rats,whereas miR-125b-1* showed no difference;serum pANCA was also up-regulated in the ulcerative colitis rats.The diagnostic performance of the established Indice =0+(2.645×miR-20b)+(1.622×miR21)+(0.979×let-7e*) + (0.082 × pANCA),which was much better than other individual markers.All the individual markers and Indice could be used effectively for surveillance of ulcerative colitis therapeutic effect,and among them Indice was the most efficient.In conclusion,circulating microRNAs is able to stably and efficiently diagnose rat ulcerative colitis and further to follow up on therapeutic effect.