The expression and roles of chemokine CCL20 in the skin lesions of psoriasis

XIE Xiangjiang; WANG Ying; CHEN Weiwen; LI Ningfei; ZHAI Chunyan; LIU Zhengrong; XIAO Zumiao; LI Ping; ZHAO Jingxia

doi:10.13431/j.cnki.immunol.j.20170090

Abstract：This study was performed to observe the expression and role of chemokine CC chemokine ligand 20 (CCL20) in the skin lesions of psoriasis.The expression of CCL20 in psoriasis patients and mice with imiquimod (IMQ)-induced psoriasis like lesions were detected by immunofluorescence;The mouse model of psoriasis was induced by adhesive tape stripping,and the severity of psoriasis was assessed according psoriasis area and severity index (PASI).After injection of recombinant mouse CCL20,the changes of psoriasis skin lesions were observed.The morphological changes of the skin tissues were observed under light microscope;the thickness of epidermis was measured.IMQ was used to induce mouse model of psoriasis,and the severity of the psoriasis was assessed by PASI after subcutaneous injection of CCL20 antibody.Real-time PCR was used to detect the expression of CCL20 in the skin tissue samples.Data showed that the expression of CCL20 in psoriasis patients and mice were increased;subcutaneous injection of recombinant mouse CCL20 united tape stripping group (CCL20 group) showed a large number of scales,erythema,infiltration and skin thickening.Compared with IMQ group,CCL20 antibody group had a lesser extent in erythema,scaling,infiltration,thickening of the epidermis and epidermal cell proliferation.Furthermore,the expression of CCL20 was significantly lower in CCL20 antibody group than that in IMQ group.In conclusion,CCL20 is highly expressed in psoriatic lesions,and recombinant mouse CCL20 can exacerbate the adhesive tape stripping induced psoriasis-like lesions in mice;subcutaneous injection of CCL20 antibody has a therapeutic effect on IMQ mice.