Biological characteristics of PHA-L-induced CIK cells and its anti-gastric cancer efficacy in combination with matrine in vitro
HUANG Mouzhen
LIU Tao
BAI Jun
TANG Baishan
LIU Yali
LI Yanhong
GAO Gui
LI Yumin
Abstract：This study aimed to explore a method to preparing cytokine-induced killer cells (CIK) by phytohemagglutinin-L (PHA-L),and to investigate the anti-tumor activity of new PHA-CIK cells combined with matrine against human gastric cancer line MGC-803.The new PHA-CIK cells were induced by PHA-L.The biological characteristics of PHA-CIK cells and CIK cells were analyzed.CCK-8 was used to detect the anti-tumor activity of the CIK cells,PHA-CIK cells,matrine and PHA-CIK cells combined with matrine (combined group) in vitro.Compared with the normal method,the new method could promote cell proliferation (P＜0.05),the prepared cell viability was kept above 90％,and CD3+CD8+ cells was increased (P＜0.05).In combined group the rate of killing MGC-803 cells was 1.6 times and 2.3 times higher than that of PHA-CIK group and matrine group,respectively (P＜0.01).Taken together,the new PHA-CIK combined with matrine has significantly improved anti-tumor activity against gastric cancer cells,and provide a new treatment method for traditional Chinese medicine combined with adoptive cellular immunotherapy.
Keywords：Phytohemagglutinin-LCytokine-induced killer cellsMatrineGastric cancerMGC-803 cellsKilling activity
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 488-491 )