The effect of STAT3 overexpression on the differentiation of mouse immature dendritic cells
XU Xiangdong
YANG Hua'an
HU Su
YANG Yuxing
ZHANG Yang
SHEN Hongjun
Abstract：This study was designed to explore whether STAT3 can increase the ability of anti-differentiation in imDC.imDC isolated from mice were infected by pLVX-IRES-ZsGreen1-STAT3 lentivirus,and then TNF-α was added into the culture.The expression of surface molecules markers CD86/MHC Ⅱ[were checked by flow cytometry.Western blot was used to detect the expression of IL-10/IDO/NF-κB;scanning electron microscopy was applied to observe ultrastructure changes of imDC;while transwell assay was used to test the cell migration of imDC.The ability of stimulating T lymphocytes was detected by mixed lymphocyte reaction.Data showed that compared with normal imDC cells,the imDC cells overexpressing STAT3 after TNF-α stimulation demonstrated significant lower expression of CD86/MHC Ⅱ,higher expression of STAT3/IL-10/IDO/NF-κB,strengthened migration ability,and weakened ability of T lymphocyte stimulation.The above results suggest that overexpression of STAT3 can increase anti-differentiation ability of imDC.
Keywords：STAT3Immature dendritic cellsImmune tolerance
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 482-487 )