Dissolving microneedles patches for whole inactivated influenza virus vaccine
Abstract：This study was performed to evaluate the immune efficacy of influenza vaccination dissolving microneedles.We applied excipients liquid and inactivated influenza virus to the mold surface and administered vacuum to pull the solution into the microneedles mold and form the microneedles.After drying,the patches was incubated in PBS to determine hemagglutinin (HA) titers.The morphological integrity of the virus particles were examined by transmission electron microscopy,and particle size distribution was determined by similarly dissolving virus solution from the patches and analyzing by dynamic light scattering.The residence time of influenza vaccine was examined by imaging in vivo.Serum samples were collected 2 weeks after vaccination for serological analysis.Serum samples were analyzed for hemagglutination inhibition (HI) activity.The results showed that the dissolving microneedles of influenza vaccine were able to be inserted into porcine skin with gentle force applied by the thumb and induce efficacy immune response.
Keywords：MicroneedlesDissolvingInfluenza vaccine
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 477-481,487 )