Calcitonin gene-related peptide inhibits the function of macrophage through inhibiting inflammasome activation
LIU Yuan
GUO Junfeng
CAI Jun
YANG Yang
LI Xing
ZHANG Huiyu
ZHANG Gang
Abstract：This study was designed to explore the calcitonin-gene-related peptide (CGRP) mediated mechanism in repressing the secretion of inflammatory factors in mouse macrophage cells.Macrophages RAW264.7 were treated by different concentrations of CGRP,then the mRNA levels of interleukin-1 β (IL-1β),tumor necrosis factor-α (TNF-α) and nicotinamide adenine dinucleotide phosphate oxidases-reactive oxygen species-NOD like receptor protein 3(NLRP3) were detected by quantitative real-time PCR (qRT-PCR),and the secretion levels of IL-1β and TNF-α were analyzed by enzyme linked immune sorbent assay (ELISA),while the protein level of NLRP3 was determined by Western blotting.Data showed that CGRP dose-dependently reduced the mRNA levels of IL-1β,TNF-α and NLRP3 in macrophages,and also reduced the protein levels of IL-1β,TNF-α and NLRP3 in the cultural supernatants.CGRP attenuated LPS-induced expression of IL-1β and TNF-α in RAW264.7 cells.Taken together,CGRP can repress the secretion of inflammatory factors in mouse macrophage cells,and the mechanism is related with the inhibition of NLRP3 expression and activity.
Keywords：CGRPNLRP3Inflammatory factorsMacrophage
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 400-404 )