The influence of SOCS3 overexpression on Th17 response in a mouse model of chronic Pseudomonas aeruginosa lung infection
DING Fengming
CHEN Yuqing
LIAO Ruomin
ZHANG Xue
FEI Xia
ZHANG Min
ZHANG Xingyi
Abstract：This study aimed to investigate the changes of Thl7 response when SOCS3 was overexpressed in CD4 + T cells isolated from a mouse model of chronic Pseudomonas aeruginosa lung infection.A recombinant lentivirus carrying SOCS3 gene was constructed and transfected into CD4+ T cells isolated from the spleens of mouse model.The SOCS3 overexpression in cells was confirmed by Western blotting and real-time PCR.Then the CD4+ T cells were stimulated with IL-23,and the expression of p-STAT3 was examined by Western blotting.The mRNA level of RORγt was examined by real-time PCR.The number of IL-17+ cells was analyzed by flow cytometry,and the level of IL-17 was assessed by ELISA.Data showed that SOCS3 overexpression significantly downregulated the levels of p-STAT3 protein and RORγt mRNA in CD4+ T cell;the number of IL-17+ cells and the level of IL-17 protein was significantly decreased in SOCS3-overexpressing cells compared with controls.These results of the in vitro study indicated that the overexpression of SOCS3 could suppress the Th17 response in mice with chronic Pseudomonas aeruginosa lung infection.
Keywords：SOCS3Pseudomonas aeruginosaChronic lung infectionTh17Neutrophil
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 389-393,399 )