Immunological effects of a novel self-assembled nanoparticle vaccine containing HIV-1 Tat49-57/HPV16 E749-57 fusion peptide and GM-CSF DNA
ZHANG Yue
XUAN Xiuyun
HAO Fei
TANG Jun
Abstract：We prepared and identified the Tat-E7/pORF-mGM-CSF nanoparticles vaccines previously,which has good antitumor effect in vaccined mice,but its specific mechanism is still unknown.In this study,we intend to find the molecular mechanism that how the vaccine induces T cell response.We detected the functions of inducting T lymphocyte proliferation,differentiation,antigen specific CTL production mediated cytotoxicity in vaccined mice.Homogeneous and dense particles with approximate diameters of 20-80 nm were observed by TEM.mGM-CSF could express in lymph nodes at the injection site and skin tissue in mice,which proved that the nanoparticles vaccine could carry exogenous genes into mammalian cells.The all results of proliferation assays,ELISPOT assays and intracellular cytokine staining and flow cytometry show that the vaccine could induce the effect of antigen specific CTL response.In conclusion,the Tat-E7/pGM-CSF vaccine can generate substantially improved epitope-specific immunity ex vivo and in vivo.
Keywords：HPVNanoparticleHIV Tat49-57GM-CSF
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 383-388 )