Establishment of ELISA kit for HCMV IgG antibody
CHEN Wujiuan
HE Meirong
YANG Rong
LI Na
FAN Weiping
Abstract：This study was performed to establish and evaluate a ELISA kit for detecting PP65 specific antibody IgG of human cytomegalovirus.After prokaryotic expression and purification of recombinant PP65322～561 protein,the ELISA plate was coated,the test conditions were optimized and adopted,then the indirect ELISA kit for detection of HCMV-IgG was established and the precision of the kit was assessed.The specificity,sensitivity and coincidence rate of the kit were analyzed through compared with an imported HCMV-IgG indirect ELISA reagent in detecting 100 samples.The coefficients of variation within assay and between assays were all lower than 10％;no cross reaction was detected in other herpes virus infecting serum.The coincidence rate of the constructed kit to the imported reagents achieved 95％.In conclusion,the constructed method shows higher specificity,sensitivity,stability and repeatability,and it provides a new method for epidemiological survey of HCMV infection.
Keywords：HCMVPP65ELISAIgG
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 349-353 )