The anti-inflammatory efficacy of Dihydromyricetin in APP/PS1 transgenic mice and its mechanism
FENG Jie
HE Guiqiong
Abstract：This study performed to observe the effect of dihydromyricetin (DHM) on the phenotypes of microglia activation and the generation of inflammatory molecules in APP/PS1 double transgenic mice.In this study,BV2 cells and APP/PS1 double transgenic mice were employed.Western blot was used to observe the expression of TNF-α in APP/PS1 mice and BV2 cells,while M1 and M2 phenotypes of microglia were detected by the immunofluorescence staining and Western blotting.Data showed that compared with control groups,DHM treatment could significantly reduce the expression levels of TNF-α,inhibit the expression of nitric oxide synthase (iNOS) and up-regulate arginase-1 (Arg-1),which suggesting that DHM may be converting M1 into M2 phenotypes.In conclusion,DHM can inhibit the inflammatory response in APP/PS1 double transgenic mice,and the mechanism may be associated with the transformation of inflammatory M 1 microglia to anti-inflammatory M2 cells.
Keywords：Alzheimer's diseaseDihydromyricetinMicroglia phenotypesTNF-α
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 301-305 )