The effect of ethyl pyruvate on proliferation of synovial fibroblasts in rheumatoid arthritis rats
LI Xiangyang
QIN Suping
YAN Chao
YU Qian
HUA Hui
PAN Wei
KONG Fanyun
ZHENG Kuiyang
TANG Renxian
Abstract：This study was performed to explore the effect of ethyl pyruvate on the proliferation and apoptosis of synovial fibroblasts.The knee synovial tissues of CIA and normal rats were collected and the synovial fibroblasts were cultured.Then the synovial fibroblasts were identified with microscope and immunofluorescence.Ethyl pyruvate of 1 mmol/L,2 mmol/L,5 mmol/L,10 mmol/L and 20 mmol/L were used to treat synovial fibroblasts,then the cell proliferation and apoptosis of the synovial fibroblasts were detected by flow cytometry/CCK8 assay and flow cytometry,respectively.Data showed that the morphology and characteristics of the cells were accorded with synovial fibroblasts,and most cells expressed vimentin.All five dosage of ethyl pyruvate could inhibit cell proliferation and induce the apoptosis of synovial fibroblasts,but not showing a dosage-dependent manner.5 mmol/ L-dosage of ethyl pyruvate could remarkably inhibit cell proliferation and induce cell apoptosis.This study suggest that ethyl pyruvate could inhibit cell proliferation and induce the apoptosis of synovial fibroblasts,and the effect is most obvious at 5 mmol/L-dosage group.
Keywords：Ethyl pyruvateSynovioblastCell proliferationCell apoptosis
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 283-287 )