Streptococcus pneumoniae initiates the formation of neutrophil extracellular traps
JIN Chunfang
HUANG Yifei
WANG Zimeng
XIANG Yun
HE Yujuan
Abstract：To investigate the effect of Streptococcus pneumoniae (S.pneumoniae) on the formation of neutrophil extracellular traps (NETs),neutrophils were isolated from mouse bone marrow by magnetic activated cell sorting,and then stimulated by S.pneumoniae for 180 minutes.NETs structure was observed by immunofluorescence staining extracellular free DNA was detected by Sytox Green staining to quantify NETs.To further understand the roles of deoxyribonuclease (DNase) and reactive oxygen species (ROS) in NETs formation,DNase and NADPH oxidase inhibitor diphenylene iodonium (DPI) which inhibits production of ROS were used.S.pn was found to be a potent inducer of NETs production and the formation of NETs was significantly decreased after being treated with DNase or DPI,which indicating that ROS was involved in the formation of NETs induced by S.pn.In conclusion,S.pn can initiate the release of NETs.DNase and DPI could be inhibitors to balance formation of NETs.
Keywords：Streptococcus pneumoniaeNeutrophilsNeutrophil extracellular trapsReactive oxygen species
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)