The therapeutic effect of (E)-phenethyl 3-(3, 5-dihydroxy-4-isopropylphenyl) acrylate gels on dermatitis eczema in mice and the mechanism
Abstract：The present study was designed to investigate the therapeutic effects of (E)-phenenthyl-3-(3,5-dihydroxy-4-isopropyl phenyl) acrylate gels (THCA354) on mouse allergic contactdermatitis (ACD) and to explore the probable mechanisms.1-fluoro-2,4-dinitrobenzene (DNFB) was daubed on the ear of mice to induce ACD model.Twenty-four hours after DNFB stimulation,by measuring the thickness of the ear and analyzing the histopathologic morphology of ear tissue,we evaluate the anti-allergic effect of THCA354 gels.The protein and mRNA expression levels of inflammatory cytokines in mouse ear tissue homogenate were determined by ELISA and RT-PCR method.Compared with negative vehicle,the ear thickness,invasion degree of lymph cell and the protein and mRNA levels of proinflammatory cytokine in ear tissue homogenate were significantly reduced,and anti-inflammatory cytokines were significantly raised in the group locally treated with THCA354 gel.In conclusion,THCA354 gels can exert anti-allergic effects by regulating the secretion and imRNA expression of inflammatory cytokines,thus to regulate the balance of the T cells subgroup.
Keywords：THCA354 gelACD modelInflammatory cytokinemRNA expression level
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)