Effects of superantigen SEB on glucocorticoid receptor in human keratinocyte
Abstract：This study aimed to investigate the effects of superantigen staphylococcal enterotoxin B (SEB) on glucocorticoid receptor in human keratinocyte HaCaT cell line.Cultured HaCaT cells were stimulated with SEB at different concentrations,and then the mRNA and protein levels of glucocorticoid receptor α and β (GRα and GRβ) were measured by RT-PCR and Western blot.After pretreated with SEB,HaCaT cells were incubated with dexamethasone for 8 h to observe the intracellular localization of GRα.Data showed that SEB could increase the mRNA and protein levels of GRβ in a dose-dependent manner,reaching a maximum when the concentration of SEB came to 100 ng/ml in HaCaT cells.Neither the mRNA nor protein level of GRα did not affected by SEB.Dexamethasone at the concentration of 10-6 mol/L had the ability to induce the translocation of GRα from the cytoplasm to the nucleus.However,the ability was partly inhibited by SEB.In conclusion,SEB may contribute to topical glucocorticoid resistance by increasing GRβ expression and inhibiting dexamethasone-induced GRα nuclear translocation in keratinocyte.
Keywords：SuperantigenSEBGlucocorticoid receptorGlucocorticoid resistance
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)