Effector memory Th9 cell phenotype and relationship with other Th cells
JIA Lei
WANG Ying
ZHANG Yannan
YU Sifei
SHEN Juan
YANG Binyan
WU Changyou
Abstract：The basic information of Th9 cells in peripheral blood of healthy persons was not fully reported.Thus,we study Th9 cell in PBMCs of healthy donors,aiming to identify the frequencies of IL-9-producing cells in PBMCs as well as Th9 cell phenotype and the relationship between Th9 cell and other Th subsets.PBMCs were isolated from healthy donors and stimulated under different conditions.ELISA,ELISpot and FACS were applied to detect the levels of IL-9 and the frequencies of IL-9-producing cells.After stimulation with anti-CD3,anti-CD3 plus anti-CD28 or PMA plus Ionomycin,the concentrations of IL-9 in culture supernatants were (21.73± 10.08) pg/ml,(74.14± 11.76) pg/ml and (94.76± 15.36) pg/ml,respectively.The frequencies of CD3-IL-9+ cells and CD3+IL-9+cell were 0.17％±0.05％ and 0.70％±0.23％.Meanwhile,the levels of CD4+ T cells (Th9 cells) were 0.51％±0.22％.Th9 cells were mainly CD45RO+CD62L+CCR7-cells (TEM cells) that were correlated with Th2 cells (IL-4+CD4+T cells) in PBMCs.Hence,we concluded that the main IL-9-producing cells of PBMCs are effecter memory Th9 cells that positively correlate with Th2 cells.
Keywords：IL-9Th9 cellMemory T cellTh cell subset
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)