Effect s of fisetin extract on mouse T lymphocytes and the NO secretion of mouse macrophages

CHEN Zhen; ZENG Yaoying; YIN Lele

Abstract：This study aims to explore the effect of fisetin (FIS) extract on mouse T lymphocytes and the NO secretion of mouse macrophages further.We used Carboxyfluorescein diacetatesuccinimidyl ester (CFDA-SE) staining combined with flow cytometry assay to obtain the proliferation-related index (PI) of lymphocytes (72 h).The expression level of CD25 of T lymphocytes stimulated with Con A was evaluated with flow cytometry.In order to detect the change of the mitochondrial membrane potential,cells were stained with 3,3-dihexyloxacarbocyanine iodide [DiOC6 (3)].ROS production was monitored by flow cytometry using 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA).Griess kit was used to evaluate the NO production of macrophages.Our results showed that fisetin (2.5 μmol/L,5 μmol/L and 10 μmol/L) significantly inhibited the proliferation of T lymphocytes.At the same time fisetin could obviously inhibit the expression of CD25 and the accumulation of ROS.We also found that the apoptosis of the lymphocytes could be accelerated by fisetin in the presence of dexamethasone (DEX).In addition,NO production of LPS plus IFN-γ-treated macrophages was clearly reduced by fisetin.These results indicate that fisetin might be a potential immunoregulation agent.