Activity of human BAFF-R gene promoter

Abstract：We would identify and analyze the promoter of BAFF-R gene 5'-flanking region, so as to define the regulation mechanism of BAFF-R expression. We cloned 5' -flanking region of BAFF-R and constructed luciferase reporter plasmids containing the 5 -flanking region of BAFF-R gene and serial deletions of the fragment. The all expression vectors were co-transfected with psv -β-gal vector into cells. The relative promoter activity of pGL3-B1, pGL3-B4, and pGL3-B7 were higher, while pGL3-B7 was the highest, as compared with others; the relative promoter activity of pGL3-B8 was the lowest in these plasmas. The results mean that transcription silence elements may exist in the region -288--430, the region -712--868, as well as the region -1420--1562. While transcription enhancer elements may exist in the region -617--712 and the region -1277--1420. Serial deletion analysis of the promoter region of BAFF-R gene suggests the sequence -1420-+261 may be a core promoter region.