Protein microarray analysis on the expression of drug resistance-related proteins in leukemic cell line
Abstract：To evaluate the value of low density protein microarray in analyzing the expression of drug resistance related proteins in leukemic cell line, we employed human leukemic cell lines (HL60,K562, HL60/VCR,K562/ADM,and Jurkat) and Burkitt lym-phoma cell line Raji in the study. The low density protein microarrays were prepared to detect the content of 11 drug resistance-related proteins in the cell lysates. The study showed that in the myeloid leukemic drug-resistant cell lines,the expression of Pglycoprotein (P-gp),lung resistance protein/major vault protein (LRP/MVP),glutathione-S-transferase-π (GST-π),vascular endothelial growth factor (VEGF), and lymphocyte function-associated antigen-1(LFA-1 )were much higher, as compared with non-drug resistant cell lines. Furthermore, in the lymphoblastic leukemic cell lines, P-gp and chemokine receptor CXCR4 were highly expressed. The results show that the protein microarray could find the diversity of drug resistance-related proteins in different leukemic cell lines. Combined detection of more than one drug resistance-related proteins is helpful to study the drug resistance mechanism of leukemia.
Keywords：Protein microarrayMultidrug resistanceLeukemic cell line
Publication Date：2010-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 333-336 )