Effects of Radix Astragali injection on CD4~+CD25~+CD127~(-/low) regulatory T cell in NOD mouse

Abstract：We aimed to investigate the effects of large does Radix Astragali injection on CD4~+CD25~+ CD127~- regulatory T cell and correlated cytokine in NOD mouse. Total of 20 NOD mice were averagely divided into 4 groups, which then administrated respectively with normal saline, cyclophosphamide injection, Radix Astragali injection, and Radix Astragali associated cyclophosphamide at the dose of 0.6 ml, for 2 weeks (successive medication). The mouse spleen was detected by flow cytometry, and found that Radix Astragali injection group and association group demonstrated significant change of regulatory T cells (P < 0.05), but blank control group or cyclophosphamide group do not (P > 0.05); we used ELISA to detect the changes of IL-10 and IL-6 in mouse peripheral blood, and found that the expression of IL-10 is significantly higher in Radix Astragali injection group and association group than that in blank control group and cyclophosphamide group (P < 0.05), while IL-6 level in the other three groups were significant lower than that in blank control group (P < 0.05). The results suggest that large dose Radix Astragali injection could suppress the proliferation and differentiation of autoreactive T cells via elevating Treg, raising IL-10, as well as reducing IL-6.