Construction and functional study of recombinant vaccine for bovine Asia I foot-and-mouth disease virus
Abstract：In this study, we tried to develop a recombinant protein vaccine for preventing bovine Asia I foot-and-mouth disease virus infection. One B cell epitope and two T cell epitopes from FMDV Vpl protein were chosen to construct genes encoding recombinant proteins RA2. And then RA2 gene (consisting BT2-BT2-BT1-BT2-BT2) was constructed by PCR and DNA cloning, which was subcloned into pET28a and highly expressed in BL21 (DE3). RA2 protein was purified by nickel chromatography and immunized in genuine pigs. ELISA and suckling mice protection method found ligh level anti-FMDV antibody in sera of the immunized genuine pigs. The results imply that the recombinant bovine Asia I foot-and-mouth disease virus vaccine RA2 may be developed as a safe and effective novel vaccine.
Keywords：Foot-and-mouth diseaseImmunityRecombinant vaccineEpitopeProkaryotic expression
Publication Date：2010-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 312-315 )