Actively invaded Plasmodium yoelii sporozoites induce DC maturation via circumsporozoite protein in vitro
Abstract：In this paper, we observe the interaction of Plasmodium yoelii sporozoites with bone marrow-dendritic cells, and investigate whether sporozoites could induce DC maturation. Dendritic cells (DCs) were induced by GM-CSF from mouse bone marrow in vitro, and co-cultured with sporozoites dissected from Plasmodium yoelii-infected A nopheles stephensi. Different time later, the interaction betwoen the DCs and sporozoites were observed under fluorescence microscope: at 2 h, sporozoites were found to be adhered on DCs; at 4 h, most sporozoites were found to be inside the DCs and keep its fusiform shape; at 24 h, most sporozoites were observed in DCs and transformed into spherical EE (early erythrocytic stage)-like forms. Although the expression of costimulatory molecules (MHC-Ⅱ ,CD80,CD86) on DCs was not affected as early as 2 h, sporozoites significantly up-regulated the expression of costimulatory molecules on DCs after 4 h and 24 h incubation with DCs. Interestingly, transfection of recombinant plasmid pFLAG-CMV8-CSP alone could also induce the expression of costimulatory molecules on DCs. The results showed that most sporozoites can actively entry into DC and induce DC maturation in vitro, while CSP might play an important role in DC maturation induced by sporozoites.
Keywords：Plasmodium yoelii sporozoitesDendritic cellPreerythrocytic stageCircumsporozoite protein
Publication Date：2010-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 294-298 )