Primary identification of H-2K~k-restricted CTL epitopes from mouse hepatitis virus
LONG Haixia
CHEN Xiaoming
WANG Yuanqiang
LIN Yong
NI Bing
ZHU Bo
WU Yuzhang
LIN Zhihua
Abstract：O bjective We identified H-2K~K-restricted CTL epitopes from mouse hepatitis virus strain 3,and the present study wouId benefit to the application of specific immunotherapy based on hepatitis virus derived epitope.Supermotif,quantitative motif combined with artificial neural networlc (ANN)and molecular modeling were used in the prediction of H-2K~k restricted cytotoxic T lymphocyte (CTL)epitope from amino acid sequence of mouse hepatitis virus strain 3.The predicted peptides were synthesized with solid phase strategies,purifted with reverse phase HPLC,and finally identified with mass spectrometry;T2-K~K cell line was used tO de-termine the peptide binding with H-2K~K molecule.At last,four candidate peptides were predicted by supermotif combined with quan-with modemtelv affinity,and the value of fluorescence index (FI)Was 1.25 and 1.04,respectively.Our results suggested that epitope prediction witll supermotif combined with quantitative motif and ANN Was consistent with peptide binding assay.In this study,both topes from MHV-3.
Keywords：Mouse hepatitis virus strain 3:Cytotoxie T lymphocyteEpitopesH-2K~k restriction
Publication Date：2010-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 161-166 )