Effects of Bai He Gu Jin Tang Drug-serum on expression of PRR in macrophages
WANG Lixin
WANG Yi
Abstract：To investigate the effects of Bai He Gu Jin Tang Drug-serum on expression of pattern recognition receptor (PRR) in macrophages for evaluating the possible mechanism of Bai He Gu Jin Tang Drug against tuberculosis. We prepared the Bai He Gu Jin Tang Drug-serum and blank serum. The two serum samples were used to incubate with U937 monocyte-derived macrophages for 24 hours. Then,RT-PCR and FACS were used to detect the mRNA expression of CR1, CR3, CD14, CD43, TLR2 and TLR4 in macrophages. Compared to blank serum group, the Bai He Gu Jin Tang drug-serum group significantly increased the mRNA and protein expressions of CR1, CD14,TLR2 and TLR4 in macrophages (P < 0.05), and up-regulated the CR3 protein expression (P<0.05) while its mRNA exprcssion didn't change. But the expression of CD43 protein and mRNA were no significant differences between the two goups (P>0.05). All the results come to the conclusion that Bai He Gu Jin Tang maybe treat the tuberculosis by regulating these PRRs in macrophages.
Keywords：Bai He Gu Jin TangMacrophagesPattern-recognition receptor
Publication Date：2010-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 148-151 )