Selection of H5N1 subtype avian influenza virus demonstrating high pathogenicity for mice
DONG Mei
GU Min
CAO Junping
PENG Daxin
WANG Xiliang
LIU Xiufan
Abstract：As HSN1 avian influenza virus can infect mammalian, research of vaccine against HSN1 virus became urgent. Our study aimed to examine the infectious status of recent H5N1 viruses against Balb/c mice and select a highly pathogenic H5N1 virus.We inoculated mice were intranasally with 17 strains H5N1 viruses isolated from poultry in southern China. The virulence and lethali-ty were observed, while TCID_(50) EID_(50), mice LD_(50) and virus loading were measured; the phylogenetic tree was drawn through HA se-quence analysis. Among the 17 H5NI strains, 1 was highly lethal to mice (TCID_(50)/100 μl = 7.2, EID_(50)/100 μl=8.325, LD_(50)/100 μl=6.318≈10~2EID_(50)). Avian influenza virus NS segments were detected from lung, spleen, nose, and brain tissues of infected mice. The vi-ral load in brain tissue was the highest, which was up to 3.56×10~(10)copies/5 μl. These results indicate that most of H5N1 viruses from poultry are nonpathogenic for mice; this mouse model can be used to evaluate the cross-protection of avian influenza vaccine and also to investigate the prophylaxis and treatment of H5N1 avian influenza viruses.
Keywords：H5N1 strainPoultryPathogenicityMice
Publication Date：2010-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 132-135,143 )