Variation and function of ERK in ALR-mediated immunosuppression
WANG Xinguo
LIU Qi
SUN Hang
XIE Songli
Liang Shan
PENG Mingli
CHEN Xuehua
Abstract：Augmenter of liver regeneration (ALR) can promote proliferation of hepatocytes, as well as plays as an immunologic sup-pressor factor. The study aims to investigate the effects of ALR on the expression and function of ERKS in the PBMC proliferation and evaluate the relevant mechanism. PBMCs were isolated from healthy volunteers by gradient centrifugation. While a series of time and concentration of ALR were administrated for optimizing the time for cells proliferation stimulated with ConA (5 ug/ml) and the ALR dose to inhibit cell proliferation. The ERK and its phosphorylation degree were evaluated by Western blot. The optimal time for ConA stimulating proliferation was 60 h, and the optimal dose of ALR to inhibit cell proliferation was 30 ug/ml. And ALR inhibited cells pro-liferation in a dose-dependent manner. But ALR did not stimulate monocytes to proliferate. The ConA increased the content and phos-phorylation of ERK significantly, but these increases were inhibited by ALR, especially the phosphorylation of ERK2. All these results suggest that ALR can inhibit the proliferation of PBMCs, probably by decreasing the total content of ERK and phosphorylation of ERK2.
Keywords：Augmenter of liver regenerationERKPBMCImmunosuppression(CSTC2008BB5228)
Publication Date：2010-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 128-131 )