Selection and identification of the peptides specifically binding to lung cancer by using phage display in vivo
PAN Qin
SHI Lei
PAN Xuediao
FENG Binghong
WU Wei
WANG Sujun
WANG Guixiang
WANG Laiyou
JIN Guifang
ZANG Linquan
Abstract：To obtain the polypeptides specifically binding to lung cancer from peptide libraries by using in vivo phage display technology.Nude mice were inoculated with human lung tumor cells A549 for setting up tumor-bearing nude mouse model.The Ph.D.-12 Phage Display Peptide Library was injected intravenously via tail vein, and 15 min after the injection, the mice were sacrificed and the phage was rescued from tumor tissues.The phage recovered from the tissues was amplified and purified, and then re-injected for next round screening.After 3 rounds of in vivo screening, the phage peptides homed to the tumor tissues was obtained.Then the phage clones were preliminary identified by cell enzyme- linked immunosorbent assay (ELISA) on lung cancer cells affinity and specificity.The positive phage clone was amplified and sequenced.Then the peptide was synthesized by chemical methods and identified for the affinity and specificity to lung cancer cells and tissues.The 20 phage clones were identified by ELISA, one of them was specially bound to the A549 cell line; the amino acid sequence was deduced and the peptide was synthesized, and named zp2, which were specially bound to the A549 cell line and the lung cancer tissue.All the results lay the foundation for the development of diagnostic reagents and drugs of lung cancer.
Keywords：Phage display in vivoA549 cell linePhage random peptide librarySpecific binding peptide
Publication Date：2010-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 68-71 )