MKL1 expression and its role in megakaryocyte differentiation
LUO Qing
SONG Guanbin
Abstract：To understand the role of MKL1 in megakaryocyte differentiation and maturation.We set megakaryocyte differentiation of mobilized human peripheral blood CD34~+ cells as studying model.Real-time quantitative PCR was used to investigate the relative MKL1 gene expression; lentivirus over-expressing MKL1 gene was constructed and used to infect PB CD34~+ cell.Cytospin and flow cytometry was used to study the morphology, CD41~+ expression, and DNA content.In mature megakaryocytes, the MKL1 gene expression was 6.8-fold higher than that in PB CD34~+ cells, meanwhile more than 2-fold higher than that in mononuclear megakaryocytes.The over-expression of MKL1 in PB CD34~+ cells promoted the differentiation and maturation of megakaryocytes in terms of CD41~+ cells and polyploidy cells (61.5% and 52.9%, contrasting to 36.3% and 33.4% in control group).We come to the conclusion that MKL1 gene expression is upregulated during the differentiation and its enhanced ectopic expression promotes the differentiation and maturation of megakaryocytes.
Keywords：MKL1MegakaryocyteMobilized human peripheral blood CD34~+ cellDifferentiationPolyploidy
Publication Date：2010-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 34-39 )