Development of immunosorbent Bio-barcode assay for the determination of the gonyautoxin_(2,3)
TANG Yong
XIANG Junjian
CHEN Yaoqiang
HE Wenfei
WANG Hong
DENG Ning
YANG Hongyu
Abstract：Objective To establish nanosphere's bio-barcode technology which can detect gonyautoxin_(2,3)(GTX_(2,3))based on the monoclonal antibody against GTX_(2,3) by using nanopaticle(NP)gold technology.Methods Aaturated ammonium sulphate method (SAS),caprylic-ammonium sulphate method(CAS),and protein A affinity chromatography were used to purify mouse ascites.The conjugation of McAb and NP was successfully prepared after determination of the best conjugation pH and minimum protein concentration.Basing on the conjugation of McAb and NP,bio-barcode DNA was labeled on the conjugation to form a McAb-NP-DNA complex.Then a novel immunosorbent bio-barcode assay combining the PCR with principle of Idc-ELISA which translating the enzyme signal to DNA signal were established based on the McAb-NP-DNA complex.Results McAb against GTX_(2,3) and NP were successfully conjugated with the conditions of pH8.5 and protein concentration on 0.096 mg/mL.GTX_(2,3) McAb-NP-barcode DNA complex was successfully prepared,thus the immunosorbent bio-barcode DNA detection assay was established,which could detect 0.74μg/mL GTX_(2,3) Conclusion It is developed that a high sensitive immunosorbent Bio-barcode assay for the determination of GTX_(2,30) in this study.
Keywords：Gonyautoxin_(23)Nanopaticle goldBio-barcode DNA
Publication Date：2009-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 713-717 )