Cloning, expression, and purification of silkworm(Bombyx mori)tropomyosin gene and identifying its immunological characteristics
LIU Zhigang
WU Yulan
YANG Rui
WU Haiqiang
Abstract：Objective To clone and express the gene of silkworm(Bombyx mori)tropomyosin,and detect the immunological activity of silkworm tropomyosin.Methods Tropomyosin of silkworm was amplified from total RNA by RT-PCR.The length of the silkworm tropomyosin fragment was 858 bp including an open reading frame coding for 286 amino acids.The recombinant silkworm tropomyosin was expressed in Escherichia coli B121(DE3)after vector PET28a transfection,and then the recombinant protein was purified by affinity chromatography with Ni~(2+) coupled to sepharose.Results Total of 5 out of 19 silkworm-allergic patients presented specific IgE antibodies to recombinant silkworm tropomyosin.Conclusion Tropomyosin gene of silkworm was cloned and expressed,which was detected as an allergen of silkworm.
Keywords：Bombyx moritropomyosinexpressionpurification
Publication Date：2009-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 655-658 )