Immune regulatory effects of genistein on animal model of rheumatoid arthritis
QIAO Lijun
WANG Yue
ZHOU Lamei
XIA Weijun
Wu Xuesu
SUN Mingxia
MA Guquan
GUO Yunke
YE Lihong
Abstract：Objective To explore immune effects of genistein on rheumatoid arthritis(RA).Methods The Ⅱ collagen-in-duced arthritis(CIA)model was established with mice;mice were devided normal group,model group,high-dose group genistein,mild-dose genistein group,low-dose genistein group,and glucosidorum tripterygll totorum group.The CIA mice were invaluated for observing T lymphocyte proliferation,T lymphocyte adhesion ability,primary synovial cell proliferation,and effects of cell culture supernatants on MMP2 and TIMP-2 expressions in spleen cells.Results In mid/high-dose genistein group,under the conditions of RPMI-1640 medium and ConA stimulating,all detecting indexes were significantly decreased,including spleen cells stimulation index,level of spleen cell adhesion to collagen type Ⅱ,sensitized T-lymphocyte adhesion ability/percentage,the primary synovial cell proliferation ability,and levels of IL-1β and TNF-α in synovial cell culture supematant.Genistein allso could step-up the expression of MMP2 and cut down the expression of TIMP-2 on the spleen cells of the CIA mice.Conclusion Genistein plays immune regulatory role in RA treatment,with better clinical effects but lower side-effects.
Keywords：genistein rootrheumatoid arthritisimmune functionexperimental research
Publication Date：2009-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 646-649,654 )