Interleukin-18-induced transdifferentiation in renal proximal tubular cells is mediated by activation of JNK pathway
YAO Cuiwei
ZHANG Junfeng
LIU Huafeng
LIANG Dong
TANG Deshen
CHEN Xiaowen
Abstract：Objective To explore the effect of JNK signaling pathway on interleukin-18(IL-18)-induced transdifferentiation in renal Droximal tubular cells.Methods Human proximal tubular epithelial cell line(HK-2 cells)was cultured in vitro.After preincubation with SP600125(0,5,10,20μmol/L)for 30 minutes,cells were exposed to IL-18(100ng/mL)for 24,48,and 72 h,respectively.The expressions of α-SMA in cultured HK-2 cells were assessed by RT-PCR and ELISA.Results SP600125 could obviously inhibit the IL-18-induced expressions of α-SMA mRNA and protein in HK-2 cells in a dose-dependent manner(P＜0.05).Conclusion IL-18-induced transdifferentiation of renal tubular epithelial cells is suppressed obviously by blocking JNK signaling pathway,thus IL-18-induced transdifferentiation of renal tubular epithelial cells is probably mediated,at least in part,by the activation of JNK signaling pathway.
Keywords：renal interstitial fibrosisrenal tubular epithelial cellsinterleukin-18transdifferentiationJNK signaling pathway
Publication Date：2009-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 629-631,637 )