Expression of B7 costimulator in human malignant hematological cell lines and its effects on activation of human peripheral blood T lymphocyte
XIONG Xiujuan
YANG Ming
FAN Dongmei
ZHAO Yingxin
JI Qing
WANG Jinhong
SHAO Xiaofeng
XU Yuanfu
XIONG Dongsheng
ZHOU Yuan
YANG Chunzheng
Abstract：Objective To explore the expression of B7 co-stimulator in human malignant hematological cell lines and its effects on activation of human peripheral blood T lymphocyte.Methods The expressions of B7 and HLA molecules on fresh hematological cell lines and on the Ara-C exposed hematological cell were detected by FAC scan cytometer.IFN-γmRNA in Ara-C treated hematological cells was detected by reverse transcription PCR(RT-PCR).Results Human malignant hematological cell lines HL-60,NB4,Jurkat,U937,Raji,Daudi,and K562 weret all expressed high levels of HLA molecules and B7-2 gene,but low or undetectable level of B7-1 gene.Ara-C treated hematological cells could stimulate the proliferation of peripheral blood mononuclear cells and promote the IFN-γproduction.Conclusion Multiple human malignant hematological cell lines express low or undetectable level of B7 gene,suggesting that B7-1 plays a critical role in tumor immunity.
Keywords：B7 costimulator moleculeantigenHLAflow cytometryT lymphocytegene expression
Publication Date：2009-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 621-624 )