Purification and characterization of glyceraldehyde-3-phosphate dehydrogenase from Lactoba.cillus acidophilus
PAN Qu
CONG Yanguang
HOU Rui
CHEN Zhijin
HU Fuquan
QIU Yue
WANG Guangke
Abstract：Objective To purify and characterize glyceraldehyde-3-phosphate dehydrogenase (GADPH) from L. acidophilus ATCC 4 356. Methods GADPH was purified through procedures of ammonium sulphate fractionation, affinity chromatography, anion-exchange, and sieve chromatography. Then the purified product was characterized by N-terminal sequencing. The apparent molecular weight of homogenous GADPH was measured by molecular sieve. Results The purified product showed a single band of 3.7×104 and was identified as GADPH of L acidophilus by N-terminal sequencing. The apparent molecular weight measured using molecular sieve was 7.08×104, which indicated the GADPH was dimeric. Conclusion The homogenous GADPH of L. acidophilus could be abundant-ly obtained by the purified program, which lays a foundation for the study of its immune function and using as an immune enhancer in the future.
Keywords：Glyceraldehyde-3-phosphate dehydrogenaseLactobacillus acidophiluspurificationN-terminal sequencingdi-mer
Publication Date：2009-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 461-464 )