Relationship of IL-6 and IL-8 secretion in epithelial ovarian cancer cell lines with their sensitivity to chemotherapy or the expressions of drug resistance-associated genes and apoptosis-inhibiting genes
WANG Yue
NIU Xiulong
GUO Xiaoqin
WU Jian
ZHU Yaqin
MAO Liqun
QU Ye
LI Tan
Abstract：Objective To investigate the relationship of IL-6 and IL-8 secretion in four epithelial ovarian cancer cell lines (A2780, CAOV-3, SKOV-3 and ES-2) with their sensitivity to chemotherapy or the expression of several drug resistance-associated genes and apoptosis-inhibiting genes. In an attempt to provide a basis for further investigating the mechanism of chemotherapy resistance caused by IL-6 and IL-8 in ovarian cancer cells. Methods Reverse transcription polymerase chain reaction (RT-PCR), enzyme linked immunosorbent assay (ELISA) and Western blot analysis were performed to analyze the expression of IL-6, IL-8, and their re-ceptors. MTT assay was carried out to examine the sensitivity to cisplatin and paclitaxel in ovarian cancer cells. The expressions of drug resistance-associated genes and apoptosis-inhibiting genes were detected by RT-PCR. Results Four ovarian cancer cells, except A2780 cells, constitutively expressed IL-6 and IL-8. The mRNA levels of IL-6 and IL-8 resembled their respective protein levels in four ovarian cancer cells. Four cell lines all expressed IL-6R, gpl30, IL-SRA, and IL-8RB. The sensitivity to cisplatin and paclitaxel in four ovarian cancer cells was different. A2780 cells were the most sensitive cells and ES-2 cells were less, whereas CAOV-3 and SKOV-3 cells were drug-resistant. The expression levels of drug resistance-asseciated genes and apoptosis-inhibiting genes were lower in A2780 and ES-2 cells, as compared with CAOV-3 and SKOV-3 cells. Conclusion Autocrine levels of IL-6 and IL-8 (especially IL-6) in epi-thelial ovarian cancer cell lines are inversely associated with their sensitivity to cisplatin or paclitaxel, and are in agreement with the ex-pression levels of several drug resistance-associated genes andapoptosis-inhibiting genes in these cells.
Keywords：IL-6IL-8ovarian cancer cellschemosensitivi-tydrug resistance-associated genesapoptosis-inhibiting genes
Publication Date：2009-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 436-441 )