rhCSa induces apoptosis by stimulating and regulating the expression of calpain in endothelial cells
HE Houxiang
LI Baosheng
LIU Li
CHEN Caiyu
LA Fenglin
Abstract：Objective To investigate the apoptotie effects of CSa on vascular endothelial cells (VECs). Methods The VECs were treated with rhCSa at different concentration (0.2, 0.5, 1 and 1.5 μg/mL, respectively) for 12 hours or with 1 μg/mL rhC5a at different time (0, 2, 6, 12, 18, and 24 h). Flow cytometry analysis with Annexin V/PI staining was used to identify the undergoing apoptosis and necrosis of VECs after rhC5a treatment. The calpain-1 and calpain-2 proteins were detected with Western blotting. Re-suits The percentage of apoptofic cells were 4.58%, 7.87%, 17.94% and 19.03%, respectively after VECs treated with rhC5a at 0.2, 0.5, 1, and 1.5 μg/mL respectively for 12 hours. Apoptotic cells were 4.58%, 12.78%, 18.12%, 19.08%, and 19.96% re-spectively in VECs treated with 1 μg/mL rhC5a for 2, 6, 12, 18 and 24 h. Western blotting shown the calpain-2 protein was time-de-pendently induced by rhCSa, however, the calpain-1 proteins were dose-dependently induced by rhC5a. Conclusion Calpain contrib-utes to cellar injury in VECs with rhC5a.
Keywords：endothelial ceilsapoptosisrhC5acalpain
Publication Date：2009-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 424-427 )