Vasoactive intestinal peptide modulates the immune function of LPS-stimulated dendritic cells
CHEN Xueqing
ZHANG Qiuhong
LIU Side
XIAO Bing
ZHANG Yali
JIANG Bo
LI Mingsong
Abstract：Objective To investigate the modulating effects of vasoactive intestinal peptide (VIP) on the immune function of LPS-stimulated dendritic cell. Methods DCs were prepared from bone marrow of C57BL/10.A with rmGM-CSF and rmIL-4, and then stimulated with different stimuli for different times. The stimulated DCs and supematant were collected for ELISA analysis; total RNA was extracted from the stimulated DC and analyzed by RNAse protection Assay. Results VIP significantly inhibited LPS-stimulated DC to produce IL-2, IL-12, TNF-α (P<0.05), MIP-2, and IP-10 (P<0.05), but the down-regulation of MIP-1α, MIP-1β, and Rantes produced by LPS-stimulated DC was not significant (P>0.05). Conclusions VIP negatively modulates the immunity of LPS-stimulated DC.
Keywords：Vasoactive intestinal peptidedendritic cellimmunity
Publication Date：2009-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 406-408 )