The relationship between juvenile idiopathic arthritis inflammatory reaction and inherent cholinergic system in dendritic cells
YANG Yang
QIU Yuzhen
SHANG Guinian
WANG Hongwei
Abstract：Objective To investigate the association of inherent cholinergic system in dendritic cells with juvenile idiopathic arthritis (JIA) inflammatory immunoreaction. Methods Bone marrows (BM) -derived DCs from healthy mouse were cultivated by in-ducing and differentiating with cytokine IL-4, GM-CSF, and LPS to maturity in vitro. The DCs were then evaluated by light microscope and flow cytometry. Flow cytometry was used to detect DC nAChRα7 in serum of normal serum group, JIA active stage serum group,and JIA active stage with MEC group. Meanwhile, IL-12 content in culture fluid, splenic lymphocyte proliferation, and CD69 expres-sion were also measured by ELISA, MTT, and flow cytometry method, respectively. Results The nAChRα7 expression was higher in JIA active stage serum group than that in normal blood serum group (P<0.01), however, there was depression tendency with MEC;the IL-12 content in JIA active stage serum group was higher obviously than that in normal blood serum group (P <0.01 ), and was ele-vated after MEC cultivation (P<0.05). CD69 expression and SI of splenic lymphocyte proliferation were the highest in MEC group,while higher in JIA active stage serum group, as compared with normal group. Conclusion JIA active stage serum can promote DC nAChRα7 expression, simultaneously, enhance the IL-12 expression and lymphocyte proliferation, which suggest that recovering cholin-ergic system may bea way for JIA therapy.
Keywords：dendritic cellcholinergic systemjuvenile idiopathicarthritisinflammatory
Publication Date：2009-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 389-392 )