Expression, purification and assessment of Borna disease virus nucleoproteinin Escherichia coli
HUO Kang
XIE Peng
XU Mingming
ZHANG Xiaodong
ZHAN Qunling
YANG Deyu
HU Yongbo
Abstract：Objective To express and purify recombinant Borua disease virus nucleoprotein in E. coli, analyzing it by specific monoclonal antibodies. Methods Construction expression vector pET-14b-BDVP40 containing the recombinant Borna disease virus nu-cleoprotein gene. The constructed vector which had been identified by enzyme digestion and nucleotide sequence analysis was trans-formed into E. coli BL21 (DED). After induced with IPTG, the recombinant protein was purified with His affinity chromatography and e-valuated its purity by SDS-PAGE. Finally the recombinant Borna disease virus nucleopretein was confirmed by Westem-blot. Results The recombinant plasmid was constructed correctly. SDS-PAGE was used to analysis the purity of recombination protein. Westeru-blot showed the purified protein could be used as an antigen to detect Borna disease virus nucleoprotein monoclonal antibodies. Conclusion The recombinant Borua disease virus nucleoprotein could be expressed with high performance, and the purified protein was certifica-ted in specific monoclonal antibodies, which was associated with BDV. This experiment made a foundation for further investigation on Boma Disease virus nucleoprotein and development of serology diagnosis kit.
Keywords：Borna disease virusnucleopreteinprokaryotic expression
Publication Date：2009-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 385-388 )